CRISPR-Cas: A Laboratory Manual. Jennifer Doudna

CRISPR-Cas: A Laboratory Manual


CRISPR-Cas-A-Laboratory-Manual.pdf
ISBN: 9781621821311 | 450 pages | 12 Mb

Download PDF




  • CRISPR-Cas: A Laboratory Manual
  • Jennifer Doudna
  • Page: 450
  • Format: pdf, ePub, fb2, mobi
  • ISBN: 9781621821311
  • Publisher: Cold Spring Harbor Laboratory Press
Download CRISPR-Cas: A Laboratory Manual


Free books online to read now no download CRISPR-Cas: A Laboratory Manual ePub by Jennifer Doudna 9781621821311

Overview

CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.



Pdf downloads:
Buenos libros para descargar FERDINAND AND ISABELLA
Free pdf books search and download Fireborne in English by Rosaria Munda
Ebook free today download Stock Trader's Almanac 2019 by Jeffrey A. Hirsch (English literature) iBook PDB PDF 9781119529316
Free books downloads The Old Drift by Namwali Serpell
Descargar libros de texto archivos pdf LA REINA DESCALZA